The canine pregnancy relaxin antigen reagent is mainly used to detect the level of relaxin dog blood or urine to assist in pregnancy diagnosis and reproductive management. It has multiple practical advantages in dog breeding management, and its main applications are as follows:

Early pregnancy screening:

Relaxin levels significantly increase in late pregnancy in dogs, and the test can identify pregnancy status early. By detecting relaxin in serum, whole blood, or urine, pregnancy can be determined at an early stage (such as 20-30 days) after dog mating, providing timely health assessment for pet owners. ‌

Miscarriage or premature birth warning: 

Dynamically monitor changes in relaxin to assist in warning of the risk of premature birth or miscarriage. Abnormal levels of relaxin may be associated with the risk of miscarriage and premature birth, and this reagent can be used to monitor pregnancy stability and assist in early intervention. ‌

Assisted diagnosis of reproductive diseases:

In addition to pregnancy testing, this reagent can also be used to assist in the diagnosis of reproductive diseases such as pyometra and ovarian dysfunction in dogs. By evaluating the synergistic changes in relaxin and progesterone levels, it provides reference for reproductive endocrine research.

High sensitivity and accuracy

This reagent can achieve early pregnancy diagnosis by detecting the level of relaxin in serum or urine (sensitivity can reach pg/mL level). The sensitivity and specificity of commercial test kits (such as Isenvo Relaxin Test) exceed 90%, making them suitable for clinical screening and effectively reducing false negative or false positive results.

Non invasive sampling and easy operation

Urine or a small amount of serum samples can be used for testing, and the sampling process is less stressful for dogs, making it suitable for family breeders to operate at home or for rapid testing in veterinary clinics. The design of formats such as colloidal gold test strips further simplifies the usage process.

Research and drug development: In the laboratory, this reagent can support the study of reproductive endocrine mechanisms in dogs and provide tools for the development of related drugs (such as drugs that regulate the reproductive cycle). ‌

Cost effectiveness and practicality

Compared to methods such as ultrasound or progesterone dynamic monitoring, relaxin reagents have lower costs and are suitable for large-scale screening or resource limited scenarios. At the same time, the results are fast (usually 15-30 minutes), making it easy to make timely decisions.

Precautions:

 There may be errors in a single test, and it is recommended to combine progesterone level monitoring (if progesterone remains above 2 ng/mL, false pregnancy can be ruled out) with dynamic observation for comprehensive evaluation. When using, follow the instructions to avoid sample contamination affecting the results.

This type of reagent usually exists in the form of test strips or Isenvo kits, with high sensitivity and specificity (some commercial reagents have a sensitivity of over 90%), easy operation, and suitable for clinical screening or laboratory testing. Attention should be paid to sample handling (such as avoiding hemolysis and ensuring fresh urine) and standard operating procedures during use to ensure reliable results.